Characterization of Mitochondrial DNA Methylation of Alzheimer’s Disease in Plasma Cell-Free DNA

Noninvasive diagnosis of Alzheimer’s disease (AD) is important for patients. Significant differences in the methylation mitochondrial DNA (mtDNA) were found AD brain tissue. Cell-free (cfDNA) a noninvasive and economical diagnostic tool. We aimed to characterize mtDNA alterations plasma cfDNA 31 patients 26 age- sex-matched cognitively normal control subjects. that patterns differed between The was predominantly hypomethylated hypomethylation sites or regions mainly located mt-rRNA, mt-tRNA, D-Loop regions. region consistent with previous studies. This study presents evidence non-protein coding may contribute pathogenesis potential application AD.